| 货号:600480 | 规格:1 ea | 目录价:¥4788 |
产品详情
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产品名称:
NAD/NADH Cell-Based Assay Kit
别名:
Nicotinamide adenine dinucleotide/Nicotinamide adenine dinucleotide, reduced
产品描述:
Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD+, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making it an important cofactor when investigating normal cellular function. Cayman’s NAD/NADH Cell-Based Assay Kit provides a colorimetric ethod for measuring intracellular NAD+and NADH in cultured cells. In this assay, NAD+ found in cell samples is reduced to NADH by alcohol dehydrogenase during the oxidation of ethanol to acetaldehyde. The newly formed and the existing NADH found in the samples is then oxidized resulting in the reduction of a tetrazolium salt substrate (WST-1) to a highly-colored formazan which absorbs at 450 nm. The amount of formazan produced is proportional to the amount of total NAD in the cell lysate and can be used as an indicator of the total cellular NAD concentration.
保存温度:
-20
运输温度:
-20
有效期:
365
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