Nucleosome, Recombinant Human, H3Cit2,8,17 dNuc, Biotinylated-突变/修饰核小体-深圳欣博盛生物科技有限公司

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Nucleosome, Recombinant Human, H3Cit2,8,17 dNuc, Biotinylated

品牌：EpiCypher

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说明书

折扣/货期

货号：16-0362

规格：50 µg

目录价：￥6900

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

Nucleosome, Recombinant Human, H3Cit2,8,17 dNuc, Biotinylated

产品描述:

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary synthetic method) contains citrulline (instead of arginine) at positions 2, 8, and 17. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group. *H3 also has a Cys to Ala substitution at position 110.

保存温度:

Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

运输温度:

Dry ice.

产品形式:

H3Cit2,8,17 dNuc (27.3 µg protein weight, 50 µg DNA + protein) in 50 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. Molarity = 5 μM. MW = 199,912.1 Da.

产地:

美国

生产商:

EpiCypher

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