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Fluorescent Probes(荧光探针)—Cayman品牌产品推荐

发布日期:2025-02-19
编辑:市场部
浏览次数:41
Cayman拥有500多种荧光探针,帮助您提升研究中所需的多功能性、灵敏度和定量能力。这一系列探针能够检测细胞内活动、蛋白质相互作用、一系列酶活性和许多其他重要目标蛋白。为实验选择最合适的荧光染料请参考以下cayman荧光探针表格。


检测细胞活力和细胞增殖的探针

Item No.

Product Name

Detects

Cell Permeable?

Excitation 

Maximum (nm)

Emission

Maximum (nm)



11397

7-Aminoactinomycin D

DNA; non-viable cells

×

488,546,578

650



14948

Calcein AM*

cell viability

494

517



20637

Calcein Blue AM*

cell viability

360

445



20632

Calcein Red™ AM*

cell viability

560

574



20639

Calcein UltraBlue™ AM*

cell viability (higher photostability   and stronger fluorescence intensity atphysiological pH than Calcein Blue)

360

445



14456

CFDA-SE

cell proliferation

491

518



20698

CytoTrace™ Red CMTPX

cell division; cell tracking

577

602



35701

Fluorescein diacetate

cell viability

490

520



19583

Green CMFDA

cell division; cell tracking

492

517



15003

JC-1

mitochondrial integrity; apoptosis

485(monomer)
  520-570(aggregate)

535(monomer)
  570-610(aggregate)





14289

Propidium Iodide

DNA; RNA; non-viable cells

×

488-535

617



14322

Resazurin (sodium salt)

enzyme activity; cell viability

570

580



21426

Tetramethylrhodamine ethyl ester   (perchlorate)

mitochondrial function; cell viability

550

575



*Also available: membrane-impermeable non-AM forms

View a complete list of cell viability fluorescent probes at www.caymanchem.com


检测核苷酸的探针

Item No.

Product Name

Detects

Cell Permeable?

Excitation

Maximum (nm)

Emission

Maximum (nm)

14338

Acridine Orange

Nucleic acids; pH

502(dsDNA)
  460(ssDNA   andRNA) 
  475 (low pH)

525(dsDNA)
  650(ssDNA and RNA) 
  590(low pH)

22439

9-Amino-6-chloro-2- methoxyacridine

DNA and pH gradients; poly(dA-dT)   sequences of DNA

411

475

11315

Chromomycin A3

DNA (two or more contiguous GC base   pairs)

×

445

575

14285

DAPI (hydrochloride)

DNA (AT-rich sequences)

×

358

461

15547

Hoechst 33342 (hydrochloride)

DNA (AT-rich sequences); nuclear   marker

350

461

14488

Pyronin Y

dsRNA

540-550

560-580

View a complete list of DNA fluorescent probes at www.caymanchem.com

 

检测氧化应激和活性反应组分的探针

Item No.

Product Name

Detects

Excitation

Maximum (nm)

Emission 

Maximum (nm)

10157

APF

hydroxyl radical; hypochlorite ion;   certain peroxidase intermediates

490

515

27086

C11 BODIPY 581/591

lipid oxidation

581/500(oxidized)

591/510(oxidized)

14051

Coumarin Boronic Acid

peroxynitrite; hypochlorous acid;   hydrogen peroxide

332

470

19111

Coumarin hydrazine

reactive carbonyl groups

365

430/550
  (upon reaction with carbonyls)

85165

DAF-2 diacetate

nitric oxide

485

538

18767

DAF-FM diacetate

nitric oxide

495

515

85155

2,7-Dichlorodihydrofluorescein   diacetate

peroxynitrite formation

502

523

12013

Dihydroethidium

superoxide

490

590

85100

Dihydrorhodamine 123

peroxynitrite formation

500

536

62237

DPPP

hydroperoxides; low-density   lipoprotein; cellular oxidation

351

380

34564

7-Fluoro-2,1,3-benzoxadiazole-   4-sulfonate (ammonium salt)

thiols

380

515

14872

Lucigenin

superoxide; chloride

455

505

16803

Luminol

peroxidation; iron; gold; cyanide

355

411

17097

Monobromobimane

reactive sulfur; thiol groups

398

490

10005983

Pentafluorobenzenesulfonyl fluorescein

hydrogen peroxide

465-505

505-555

16929

WSP-5

hydrogen sulfide

502

525

 View a complete list of fluorescent probes for oxidative stress and reactive species detection at www.caymanchem.com

 

检测细胞骨架蛋白的探针

Item No.

Product Name

Detects

Excitation

Maximum (nm)

Emission 

Maximum (nm)

21016

9-(2,2-Dicyanovinyl)julolidine

tubulin and other cytoskeletal   proteins

450

480(low viscosity solvents) 
  505(high   viscosity solvents)

20546

Phalloidin-California Red™ Conjugate

F-actin

583

605

20478

Phalloidin-Fluorescein Conjugate

F-actin

492

518

20547

Phalloidin-iFluor™ 350 Conjugate

F-actin

353

442

20548

Phalloidin-iFluor™ 405 Conjugate

F-actin

400

421

20549

Phalloidin-iFluor™ 488 Conjugate

F-actin

493

517

20552

Phalloidin-iFluor™ 555 Conjugate

F-actin

556

574

20553

Phalloidin-iFluor™ 594 Conjugate

F-actin

590

618

20554

Phalloidin-iFluor™ 633 Conjugate

F-actin

634

649

20555

Phalloidin-iFluor™ 647 Conjugate

F-actin

650

665

20545

Phalloidin-Tetramethylrhodamine   Conjugate

F-actin

546

575

View a complete list of cytoskeletal protein fluorescent probes at www.caymanchem.com 


荧光探针.png荧光探针(1).png荧光探针(2).png

Actin   filaments stained with
  Phalloidin-iFluor™ 350 Conjugate

HeLa cells   stained with
  Phalloidin-iFluor™ 488 Conjugate

HeLa cells labeled   with
  Phalloidin-iFluor™ 594 Conjugate


蛋白酶底物探针

Item No.

Product Name

Detects

Excitation

Maximum (nm)

Emission 

Maximum (nm)

9002021

Abz-Val-Ala-Asp-Nva-Arg-Asp-   Arg-Gln-EDDnp (trifluoroacetate salt)

human proteinase 3 activity

320

420

26640

Ac-ANW-AMC

β5i/LMP7 subunit of the 20S   immunoproteasome activity

340-360

440-460

14986

Ac-DEVD-AMC

caspase-3 activity

340-360

440-460

17051

Ac-LEHD-AFC (trifluoroacetate salt)

caspase-4, -5, and -9 activity

400

505

14991

Ac-LETD-AFC

caspase-8 activity

400

505

14988

Ac-VDVAD-AFC

caspase-2 activity

400

505

14992

Ac-WEHD-AFC (trifluoroacetate salt)

caspase-1, -4, and -5 activity

400

505

17591

Ac-YVAD-AFC

caspase-1 and -4 activity

400

505

27580

Gly-Arg-AMC (hydrochloride)

cathepsin C activity

340-360

440-460

14907

MeOSuc-Ala-Ala-Pro-Val-AMC

human leukocyte and porcine pancreatic   elastase activity

355-380

440-460

10008119

Suc-Leu-Leu-Val-Tyr-AMC

20S proteasome, chymotrypsin-like   proteases, and calpain activity

360

460

11675

(Z-Ala-Ala-Ala-Ala)2Rh110

elastase activity

485

525

10008117

Z-LLE-AMC

caspase-like activity of the 26S   proteasome or 20S proteolytic core

340-360

440-460

 View a complete list of protease activity fluorescent probes at www.caymanchem.com

 

其他酶底物探针

Item No.

Product Name

Detects

Excitation

Maximum (nm)

Emission

Maximum (nm)

62910

7-hydroxycoumarinyl Arachidonate

cPLA2 activity

335

450

25583

C12FDG

β-galactosidase activity

485

530

35500

DiFMUP

phosphatase activity

358

455

16551

4-Methylumbelliferyl-α-D-   Galactopyranoside

α-galactosidase activity

330(pH 4.6);

370 (pH 7.4);

385(pH 10.4)

445-454

20948

4-Methylumbelliferyl-β-D-   Glucopyranoside

β-glucosidase and β-glucocerebrosidase   activity

330(pH 4.6);

370 (pH 7.4);

385(pH 10.4)

445-454

19543

4-Methylumbelliferyl-α-L- Iduronide   (free acid)

α-L-iduronidase activity

320(pH 1.97-6.72);
  360(pH   7.12-10.3)

445-455
  (increases as pH decreases)

19524

4-Methylumbelliferyl 6-thio- Palmitate-β-D-Glucopyranoside

palmitoyl-protein thioesterase   (PPT/CLN1) activity

320(pH 1.97-6.72);
  360(pH   7.12-10.3)

445-455
  (increases as pH decreases)

 View a complete list of enzyme substrate fluorescent probes at www.caymanchem.com

 

检测蛋白交互作用探针

Item No.

Product Name

Detects

Excitation

Maximum (nm)

Emission 

Maximum (nm)

20672

3-BODIPY-propanoylaminocaproic Acid   N-hydroxysuccinimide ester

proteins immobilized on PVDF membranes

300-360,504

511

20704

FlAsH-EDT2

proteins with a tetracysteine sequence

508

528

19767

ReAsH-EDT2

proteins with a tetracysteine sequence

593

608

View a complete list of probes to detect protein interactions at www.caymanchem.com  

 

检测钙离子探针

Item No.

Product Name

Detects

Cell

 Permeable?

 Excitation

Maximum (nm)

Emission

Maximum (nm)

20424

BTC AM*

intracellular calcium (low affinity)

401(Ca2+-bound)/480

529

35758

Fluo-2 AM*

intracellular calcium

490

515

14960

Fluo-3 AM*

intracellular calcium

488

526

31143

Fluo-4 AM*

intracellular calcium

490

520

14591

Fura-2 AM*

intracellular calcium

380 (low [Ca2+])
  340 (high   [Ca2+])

510

34993

Fura-2 Leakage Resistant AM*

intracellular calcium

340 (Ca2+-bound)
  380   (Ca2+-free)

505

20416

Fura-FF AM*

intracellular calcium (low affinity)

365 (no Ca2+)
  339 (high   [Ca2+])

514(no Ca2+)
  507(high [Ca2+])

34985

ICR-1 AM*

intracellular calcium

580

660

20417

Indo-1 AM*

intracellular calcium

349-364

475-485(no Ca2+)
  400-410(Ca2+-bound)

20442

Rhod-5N AM*

intracellular calcium (low affinity)

551

576

20444

Rhod-FF AM*

intracellular calcium (low affinity)

552

580

*Also available: membrane-impermeable non-AM forms

View a complete list of calcium fluorescent probes at www.caymanchem.com

 

检测pH值探针

Item No.

Product Name

Detects

Cell Permeable?

Excitation

Maximum (nm)

Emission

Maximum (nm)

17183

BCECF

pH

×

 440 / 490

535

15922

BCECF AM

intracellular pH

 440 / 490

535


 

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