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The most prominent function of the NLRP3 inflammasome is the processing and activation of pro-interleukin-1β (pro-IL-1β). Yet most cells do not express pro-IL-1β and thus prior expression of pro-IL-1β is required. This can be achieved by stimulating receptors such as TLRs (e.g. through LPS or PMA), Nods, TNF-Rs (e.g. through TNF-α) or IL-1R1 (through IL-1α and IL-1β) that activate NF-κB and initiate pro-IL-1β transcription. This process is called priming. Priming also induces NF-κB-dependent transcription of NLRP3.

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