深圳欣博盛生物科技有限公司

CUTANA™ Quick Cleanup DNA Purification Kit

The CUTANA™ Quick Cleanup DNA Purification Kit can be used to purify CUT&RUN or CUT&Tag DNA and remove unwanted adapter-/primer-dimers from these reactions. Unlike many kits that purify DNA using spin columns, this kit uses SPRI paramagnetic beads for streamlined, higher throughput workflows easily integrated into CUTANA™ genomic mapping assays. Depending on the application, various SPRI bead:DNA ratios are added to capture target DNA. In CUT&RUN, pAG-MNase cleaves DNA proximal to antibody-labeled chromatin, releasing it into the supernatant. It is imperative to recover the highest DNA yield possible from the supernatant; therefore, a higher bead:DNA ratio is added to span a wide range of DNA fragment lengths. This kit includes sufficient volume to perform 48 total CUT&RUN reactions at an empirically optimized higher bead ratio. In CUT&Tag, pAG-Tn5 cleaves antibody-bound chromatin and simultaneously ligates adapter DNA, resulting in longer DNA fragments. Further, in the EpiCypher CUTANA™ Direct-to-PCR CUT&Tag workflow, indexing PCR is used to amplify next-generation sequencing (NGS) libraries directly from the reaction mixture prior to DNA purification. In this case, a lower bead ratio is ideal for preferable enrichment of longer DNA fragments and selective cleanup of sequencing libraries. This kit includes sufficient volume to perform 96 total CUT&Tag reactions at an empirically optimized lower bead ratio. This kit can also be used to cleanup NGS libraries by removing contaminating small fragments such as adapter dimers in CUT&RUN (~150 bp) and primer dimers in CUT&Tag (~25-75 bp).

Store at room temperature. Stable for 1 year upon date of receipt.

Room temp. Can go on frozen cold packs.

美国

EpiCypher