dCypher™ Nucleosome Full Panel-核小体套装-深圳欣博盛生物科技有限公司

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dCypher™ Nucleosome Full Panel

品牌：EpiCypher

官网链接

说明书

折扣/货期

货号：16-9001

规格：96-well plate

目录价：￥21540

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

dCypher™ Nucleosome Full Panel

产品描述:

Understanding epigenetic reader proteins and their binding preference for post-translational modifications (PTMs) is key to unveiling how these proteins regulate genome processes and how their dysregulation may be contributing to disease. The dCypher™ Nucleosome Panel enables access to epigenetic diversity in a physiologically relevant context, the nucleosome, where chromatin reader activity can be accurately interrogated. The 96-well plate contains recombinant mononucleosomes as well as appropriate controls. Human histones expressed in E. coli bearing single and combinatorial histone post-translational modifications (PTMs) are wrapped by 147 or 199 base pair DNA with a 5’ biotin-TEG group and a central 601-positioning sequence, identified by Lowary and Widom [1]. All nucleosomes in the panel are subjected to EpiCypher’s rigorous quality control metrics, including: ESI-TOF mass spectrometry analysis of the modified histones, SDS-PAGE to confirm octamer composition and purity, native PAGE to confirm nucleosome assembly, and western blot analysis of the PTM, histone mutation, or histone variant (if applicable). For the full list of nucleosomes in the panel, including individual catalog numbers of full-size (50 µg) products, see the associated excel sheet on the product page at www.epicypher.com/16-9001. Full plate layout and key:

保存温度:

Stable for six months at -80°C from date of receipt. Avoid freeze/thaws.

运输温度:

Dry ice.

产品形式:

The material included in this 96-well plate is provided at 1.5 µM in 5 µL of nucleosome storage buffer (10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT*, & 20% glycerol). *dNucs containing crotonyl-lysine are stored in nucleosome storage buffer without DTT. MW = ~200,000 Da

产地:

美国

生产商:

EpiCypher

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