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折扣/货期| 货号：16-0347 | 规格：50 µg | 目录价：￥5700 |
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
产品名称:
Mononucleosomes (H3.3G34V), Recombinant Human Biotinylated
产品描述:
Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing a valine at position 34, expressed in E. coli. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). The substitution of V for G at position 34 is associated with pediatric diffuse intrinsic pontine gliomas (DIPGs) with and diminished levels of H3K36 methylation. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group.
保存温度:
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
运输温度:
Dry ice.
产品形式:
H3.3G34V Mononucleosomes (27.3 µg protein weight, 50 µg DNA + protein) in 37.6 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. Molarity = 6.63 μM. MW = 200,621.
产地:
美国
生产商:
EpiCypher
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下一个:Mononucleosomes (H3.3K4M), Recombinant Human Biotinylated
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