货号:700160 | 规格:2 x 96 wells | 目录价:¥3556 |
产品详情
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产品名称:
Myeloperoxidase Peroxidation Fluorometric Assay Kit
别名:
MPO Peroxidation Assay Kit
产品描述:
Cayman’s MPO Peroxidation Fluorometric Assay provides a convenient fluorescence-based method for detecting MPO peroxidase activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the peroxidase component of MPO, where a single two-electron oxidation of native enzyme (MPO) to compound I (MPO-I) is followed by two successive one-electron reductions back to native enzyme by compound II (MPO-II).{12432} The reaction between hydrogen peroxide and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin. Resorufin fluorescence can be easily analyzed with an excitation wavelength of 530 nm and emission wavelength of 590 nm. The kit includes an MPO-specific inhibitor for distinguishing MPO activity from MPO-independent fluorescence.
保存温度:
4
运输温度:
4
有效期:
365
灵敏度:
0.08 U/ml
检测范围:
0-1.5 µM
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