深圳欣博盛生物科技有限公司

Heterotypic [H3K4me3 • H3K9,14,18ac] Recombinant Nucleosome, Biotinylated

Heterotypic nucleosomes, also referred to as “asymmetric nucleosomes,” contain sister histones with distinct histone variants and/or post-translational modifications (PTMs). In homotypic nucleosomes, or “symmetric nucleosomes,” each pair of sister histones bears the same PTM, set of PTMs, or histone variant. Histone-modifying enzymes, chromatin remodelers, and histone chaperones differentially modify sister histones or exchange unique histone variants to form heterotypic nucleosomes. Heterotypic nucleosomes have been found at promoters of developmental genes in undifferentiated embryonic stem cells and transcription start sites (TSSs) of approximately half of the genes in budding yeast [1]. Heterotypic nucleosomes represent an additional layer of the histone code, acting as substrates for multivalent reader proteins, participating in PTM crosstalk mechanisms, and influencing reader protein binding affinity through varying local target concentration. Recombinant heterotypic nucleosomes are useful for studying chromatin dynamics and transcriptional regulation. Heterotypic [H3K4me3 • H3K9,14,18ac] Recombinant Nucleosome, Biotinylated is a fully defined semi-synthetic nucleosome containing combinatorial PTMs in both cis and trans whereby one histone H3 is trimethylated at lysine four while the other is acetylated at lysines 9, 14, and 18. [H3K4me3 • H3K9,14,18ac] nucleosome consists of 147 base pairs of 601 sequence DNA [2] wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3.2, and H4) to form a nucleosome, the basic repeating unit of chromatin. The DNA contains a 5’ biotin-TEG group, and histone H3.2 has a Cys to Ala substitution at position 110. H3 hyperacetylation in cis (e.g. H3K9,14,18ac) increases tail accessibility for H3K4 methyl writers such as the methyltransferase MLL1, emphasizing the role of PTM crosstalk and heterotypic nucleosomes in chromatin regulatory mechanisms [3].

Human

E. coli & synthetic DNA

Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.

Dry ice.

0.7 mg/mL mononucleosome in 35.7 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol (13.6 µg protein, 25 µg DNA + protein).

EpiCypher