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GeneTex热销抗体 —beta Actin抗体

发布日期:2024-11-26
编辑:市场部
浏览次数:154
GeneTex热销的beta Actin抗体,目前有600余篇高分文献引用。beta Actin是细胞骨架蛋白之一,分子量为42 kDa,由于在各组织与细胞中表达相对恒定,因此常拿来做内参(尤其是WB实验),以估计样本中蛋白质的数量和质量。

 

产品详情

产品名称:beta Actin antibody

别名:actin beta , BRWS1 , PS1TP5BP1

反应种属:Caenorhabditis elegans, Candida albicans, Cat, Chicken, Dog, Drosophila, E. coli, Hamster, Horse, Human, Monkey, Mosquito, Mouse, Pig, Plant, Rat, Rice, Sheep, Treeshrew, Worm, Xenopus, Yeast

宿主来源:Rabbit

实验应用:ICC/IF, IHC-Fr, IHC-P, IP, WB

同种型:IgG

免疫原:Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

克隆性:Polyclonal

纯化方式:Purified by antigen-affinity chromatography.

偶联:Unconjugated

产品浓度:1.4 mg/ml (Please refer to the vial label for the specific concentration.)

保存温度:Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

运输温度:4°C

预期分子量:42

产品形式:Liquid

存储溶液:PBS, 20% Glycerol, 0.025% ProClin 300.


验证数据

GTX109639.png

WB:

Sample (30 μg of whole cell lysate)

A: 293T

B: NTH-3T3

C: mouse brain

D: PC-12

E: Rat brain

F: drosophila

10% SDS PAGE

GTX109639 diluted at 1:10000

The HRP-conjugated anti-rabbit IgG   antibody (GTX213110-01) was used to detect the primary antibody.

null

IHC-P:

beta Actin antibody detects beta Actin   protein at cell membrane and cytoplasm by immunohistochemical analysis.

Sample: Paraffin-embedded mouse   intestine.

beta Actin stained by beta Actin   antibody (GTX109639) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH   6.0, 15 min

GTX109639(2).png

ICC/IF:

beta Actin antibody detects beta Actin   protein at cytoskeleton by immunofluorescent analysis.

Sample: HeLa cells were fixed in   ice-cold MeOH for 5 min.

Green: beta Actin stained by beta Actin   antibody (GTX109639) diluted at 1:500.

Blue: Fluoroshield with DAPI   (GTX30920).

 

产品部分引用文献

1.Zhao Y et al. J Adv Res 2024; S2090-1232 (24) : 00216-9 SLC7A11 as a therapeutic target to attenuate phthalates-driven testosterone level decline in mice.

2.Y Yang et al. Int J Gen Med 2024; 2024 (17) : 1923-1935 CLEC4D as a Novel Prognostic Marker Boosts the Proliferation and Migration of Gastric Cancer via the NF-κB/AKT Signaling Pathway.

3.MW Tsai et al. J Funct Foods 2024; 116 : 106212 Alpha-lipoic acid ameliorates influenza A virus caused acute pneumonia though enhancement of anti-viral T cell immunity and suppression of macrophage activation by inhibiting ERK1/2 signaling pathway.

4.AD Do et al. J Exp Clin Cancer Res 2024; 43 (1) : 130 LOXL1-AS1 contributes to metastasis in sonic-hedgehog medulloblastoma by promoting cancer stem-like phenotypes.

 

订购详情

货号

产品名称

规格

GTX109639-S

beta Actin抗体

25 μl

GTX109639

100 μl

 


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