| 货号:GTX17588 | 规格:1kit | 目录价:¥7900 |
产品详情
* 以下信息仅供参考,详情请以原厂网站为准
产品名称:
Serotonin ELISA Kit
别名:
Serotonin,5HT,5-hydroxytryptamine
产品描述:
This immunoassay kit allows for the in vitro quantitative determination of general Serotonin concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serotonin, During the reaction, Serotonin in the sample or standard competes with a fixed amount of biotin-labeled Serotonin for sites on a pre-coated Monoclonal antibody specific to Serotonin. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serotonin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
反应种属:
Species independent
实验应用:
ELISA
保存温度:
The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20ºC upon being received. After receiving the kit , Substrate should be always stored at 4ºC.
运输温度:
-20°C
生产商:
GeneTex
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