Glutamine Fluorometric/Colorimetric Assay Kit-比色法检测试剂盒-深圳欣博盛生物科技有限公司

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Glutamine Fluorometric/Colorimetric Assay Kit

品牌：Cayman

官网链接

说明书

折扣/货期

货号：702430

规格：96 Wells

目录价：￥6300

产品详情

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产品名称:

Glutamine Fluorometric/Colorimetric Assay Kit

产品描述:

Cayman’s Glutamine Fluorometric/Colorimetric Assay Kit provides both fluorometric and colorimetric methods for measuring glutamine in biological samples such as cell culture supernatants, cell lysates, tissue homogenates, serum, and plasma. In the assay, the Glutamine Converter Enzyme catalyzes the conversion of glutamine to glutamate, which is further converted to α-ketoglutarate (α-KG) and hydrogen peroxide (H2O2) in the presence of the Glutamine/Glutamate Developer Enzyme Mix. MaxiProbe reacts with H2O2, forming a compound that can be easily quantified at excitation and emission wavelengths of 535±5 and 590±10 nm, respectively. Alternatively, the absorbance of the product can be measured at 570 nm. Background from glutamate and H2O2 in the sample is determined by omitting the converter enzyme in the sample background reactions.This assay offers the option of measuring fluorescence or absorbance. It is at the user’s discretion to choose the mode of measurement (and corresponding standard curve preparation) that best fits their needs. When read fluorometrically, this assay has a range of 0-40 μM and a lower limit of detection (LLOD) of 1.6 μM. When read colorimetrically, the range is 0-400 μM with an LLOD of 2.3 μM.

保存温度:

-20

运输温度:

-20

有效期:

365

检测范围:

0-40 µM (Fluorometric); 0-400 µM (Colorimetric)

来源:

Animal/Bovine|Bacterium/E. coli|Bacterium/Streptomyces

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