CBP bromodomain TR-FRET Assay Kit-时间分辨荧光分析试剂盒-深圳欣博盛生物科技有限公司

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CBP bromodomain TR-FRET Assay Kit

品牌：Cayman

官网链接

说明书

折扣/货期

货号：600850-1920 wells	规格：1920 wells	目录价：￥30184
货号：600850-384 wells	规格：384 wells	目录价：￥8050
货号：600850-9600 wells	规格：9600 wells	目录价：￥140882

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

CBP bromodomain TR-FRET Assay Kit

产品描述:

Cayman’s CBP bromodomain TR-FRET Assay Kit is a homogeneous, time-resolved Förster resonance energy transfer (TR-FRET) assay method amenable to rapid characterization of inhibitors of the bromodomain-acetylated peptide interaction in a high-throughput format. The donor fluorophore in this assay consists of CBP bromodomain (BRD; human; amino acids 1,081-1,197) directly labeled with a europium (Eu3+) chelate (BRD-Eu3+). A biotinylated peptide containing acetylated lysines serves as the ligand for the CBP bromodomain. Allophycocyanin (APC)-labeled avidin binds with high affinity to the peptide substrate via the biotin moiety and serves as the acceptor fluorophore in the assay. Inhibition of the BRD-peptide interaction displaces BRD-Eu3+ from the APC-labeled avidin resulting in a loss of TR-FRET signal. The CBP bromodomain TR-FRET Assay Kit is robust (Z´ = >0.6) and suitable for high-throughput screening in the provided 384-well plate or can be scaled to higher density plate formats (e.g., 1,536-well plate) if desired. The assay is stable at room temperature for at least four hours and in the presence of less than 2% DMSO.

保存温度:

-80

运输温度:

-80

有效期:

365

来源:

Animal/Bovine

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