cGAS TR-FRET Inhibitor Screening Assay Kit-时间分辨荧光分析试剂盒-深圳欣博盛生物科技有限公司

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cGAS TR-FRET Inhibitor Screening Assay Kit

品牌：Cayman

官网链接

说明书

折扣/货期

货号：702120-384 wells	规格：384 wells	目录价：￥9394
货号：702120-96 wells	规格：96 wells	目录价：￥9394

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

cGAS TR-FRET Inhibitor Screening Assay Kit

别名:

2'3'-cGAMP Synthase|C6orf150|Cyclic GMP-AMP Synthase

产品描述:

Cayman’s cGAS TR-FRET Inhibitor Screening Assay Kit provides a robust and easy-to-use time-resolved Förster resonance energy transfer (TR-FRET) platform for identifying novel inhibitors of human cGAS, which is a cystosolic dsDNA sensor that detects microbial pathogens. This assay utilizes a mouse anti-rabbit monoclonal immunoglobulin G (IgG) directly labeled with a europium (Eu3+) chelate as a donor molecule and fluorescently labeled 2’3’-cGAMP as the acceptor molecule. In the presence of an anti-2’3’-cGAMP antibody, the donor and acceptor are brought into close proximity resulting in a FRET from the donor to the acceptor upon excitation of the Eu3+-conjugated IgG at 340 nm and an emission from the acceptor at 665 nm. In the assay, production of 2’3’-cGAMP by cGAS in the presence of dsDNA, ATP, and GTP results in the displacement of fluorescently labeled 2’3’-cGAMP from the anti-2’3’-cGAMP antibody, resulting in a loss of TR-FRET signal. Inhibition of cGAS maintains the signal. The cGAS inhibitor CU-76 is included as a positive control.

保存温度:

-80

运输温度:

-80

有效期:

365

来源:

Animal/Bovine|Animal/Mouse|Animal/Rabbit

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